Part:BBa_K4803107

CD4signal-IFNGRex-CD28TMD-TEV CS-PPVp

Introduction

This biobrick is Part of MESA (Modular Extracellular Sensor Architecture) created by combining CD4 signal (BBa_K4803005), IFNGR1 extracellular domain (BBa_K4803002), CD28 transmembrane domain (BBa_K4803006), TEV cleavage site (BBa_K1362453), and PPV protease (BBa_K1965025).

Usage and Biology

This Part is based on the mechanism of MESA, a synthetic receptor. When IFN-γ is detected by this Part and another receptor part of the MESA system (BBa_K4803105), the two Parts dimerize. Upon dimerization, the TEV protease of BBa_K4803105 is activated, which cleaves the intracellular TEV protease cleavage site of this Part and releases the lower PPV protease.

Figure 1: Overview of MESA system

In UTokyo 2023 project, protease released from MESA connects to the downstream Secretion unit and Amplification unit.

Figure 2: Overview of UTokyo2023 project "SWIFT" system

Reference

Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS synthetic biology, 3(12), 892-902. https://doi.org/10.1021/sb400128g

Schroder, K., Hertzog, P. J., Ravasi, T., & Hume, D. A. (2004). Interferon-γ: an overview of signals, mechanisms and functions. Journal of Leucocyte Biology, 75(2), 163-189. https://doi.org/10.1189/jlb.0603252

https://www.ncbi.nlm.nih.gov/nuccore/NM_000416.3?report=genbank

Adams, M. J., Antoniw, J. F., & Beaudoin, F. (2005). Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Molecular Plant Pathology, 6(4), 471–487. https://doi.org/10.1111/j.1364-3703.2005.00296.x

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1243
  Illegal BsaI.rc site found at 1423