Part:BBa_K4803104

CD4signal-gp130ex-CD28TMD-TEV CS-tTA

Introduction

This biobrick is MESA Part created by combining BBa_K4803005(CD4 signal), BBa_K4803000(gp130 extracellular domain), BBa_K4803006(CD28 Transmembrane domain), BBa_K1362453(TEV cleavage site), BBa_K2696013(tTA).

Usage and Biology

This Part is based on the mechanism of MESA, a synthetic receptor. When extracellular IL-6 is detected by this Part and BBa_K4803103, the two Parts dimerize. Upon dimerization, TEV protease of this Part is activated, which cleaves the intracellular TEV protease cleavage site of BBa_K4803103 and releases the lower tTA. The protein is then transported to the endoplasmic reticulum by BBa_K4803005, a CD4-derived ER trafficking signal, and is transmembrane by BBa_K4803006, a CD28-derived transmembrane domain.

Reference

Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS synthetic biology, 3(12), 892-902. https://doi.org/10.1021/sb400128g

Hibi, M., Murakami, M., Saito, M., Hirano, T., Taga, T., & Kishimoto, T. (1990). Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell, 63(6), 1149-1157. https://doi.org/10.1016/0092-8674(90)90411-7

Taga, T., & Kishimoto, T. (1997). Gp130 and the interleukin-6 family of cytokines. Annual review of immunology, 15(1), 797-819. https://doi.org/10.1146/annurev.immunol.15.1.797

Kaur, S., Bansal, Y., Kumar, R., & Bansal, G. (2020). A panoramic review of IL-6: Structure, pathophysiological roles and inhibitors. Bioorganic & medicinal chemistry, 28(5), 115327. https://doi.org/10.1016/j.bmc.2020.115327

Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods, 19(6), 679-682. https://doi.org/10.1038/s41592-022-01488-1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 87
  Illegal BamHI site found at 905
  Illegal BamHI site found at 2623
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]