Part:BBa_K4803103

CD4signal-gp80ex-CD28TMD-TEVp

Introduction

This biobrick is MESA (Modular Extracellular Sensor Architecture) Part created by combining BBa_K4803005(CD4 signal),Part:BBa_K4803001(gp80 extracellular domain), Parts:BBa_K4803006(CD28 Transmembrane domain),Part:BBa_K2969000(TEV protease).

Usage and Biology

This Part is based on the mechanism of MESA, a synthetic receptor. When extracellular IL-6 is detected by this Part and BBa_K4803104, the two Parts dimerize. Upon dimerization, TEV protease of this Part is activated, which cleaves the intracellular TEV protease cleavage site of BBa_K4803104 and releases the lower tTA. The protein is then transported to the endoplasmic reticulum by BBa_K4803005, a CD4-derived ER trafficking signal, and is transmembrane by BBa_K4803006, a CD28-derived transmembrane domain.

Figure 1: Overview of MESA system

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 244
  Illegal NgoMIV site found at 328
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1513
  Illegal SapI.rc site found at 1861