Composite

Part:BBa_K4803102

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-gp80ex-CD28TMD-TEV CS-tTA

Introduction

This biobrick is MESA (Modular Extracellular Sensor Architecture) Part created by combining BBa_K4803005(CD4 signal), BBa_K4803001(gp80 extracellular domain), BBa_K4803006(CD28 Transmembrane domain), BBa_K1362453(TEV cleavage site), BBa_K2696013(tTA).

Usage and Biology

This Part is based on the mechanism of MESA, a synthetic receptor. When IL-6 is detected by this Part and BBa_K4803101, the two Parts dimerize. Upon dimerization, TEV protease of BBa_K4803101 is activated, which cleaves the intracellular TEV protease cleavage site of this part and releases the lower tTA.

control
Figure:The mechanism of MESA system


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None