Composite

Part:BBa_K4803101

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-gp130ex-CD28TMD-TEVp

Introduction

This biobrick is MESA (Modular Extracellular Sensor Architecture) Part created by combining BBa_K4803005(CD4 signal), BBa_K4803002(gp130 extracellular domain), BBa_K4803006(CD28 Transmembrane domain), BBa_K2969000(TEV protease).

Usage and Biology

This Part is based on the mechanism of MESA, a synthetic receptor. When extracellular IL-6 is detected by this Part and BBa_K4803102, the two Parts dimerize. Upon dimerization, TEV Protease of this Part is activated, which cleaves the intracellular TEV Protease cut site of BBa_K4803102 and releases the lower tTA. The protein is then transported to the endoplasmic reticulum by BBa_K4803005, a CD4-derived ER trafficking signal, and is transmembrane by BBa_K4803006, a CD28-derived transmembrane domain.


control
Figure 1:The mechanism of MESA system

control
Figure 2:Prediction of transmembrane domains by TMHMM


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1159
    Illegal SapI.rc site found at 1507


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Categories
Parameters
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