RBS

Part:BBa_K4701231

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-08-10)


Synthetic RBS for tphA2

RBS designed for use in the synthetic operons added as composite parts BBa_K4701306, BBa_K4701307, BBa_K4701308. The operon calculator, which is a biophysical model taking into account translational coupling in operons was used to generate the initial designs and validate further tweaks [1].

References

1. Tian T, Salis HM. (2015) A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons. Nucleic Acids Research. 43(14), 7137–7151. https://doi.org/10.1093/nar/gkv635

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None