Composite

Part:BBa_K4701308

Designed by: Henri Sundquist   Group: iGEM23_Aalto-Helsinki   (2023-08-30)


Synthetic tpaK-tphII operon (Low transcription)

This part is identical to BBa_K4701306 apart from the promoter, which is BBa_J23114, as it has been observed to enable lower expression in Pseudomonas putida [1].

See the part page of BBa_K4701306 for discussion about the utility of this synthetic operon.

References

[1] Pearson, AN. et al. (2023) The pGinger Family of Expression Plasmids Bond DR, editor. Microbiology Spectrum. 11(3). http://doi.org/10.1128/spectrum.00373-23


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5334
    Illegal SapI site found at 5291


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