Coding
TAT-Ftn

Part:BBa_K4669005

Designed by: Lisa Siemers   Group: iGEM23_Hamburg   (2023-09-30)


TAT-Ferritin
Function Coding sequence
Use in Bacterial cells
RFC standard RFC 10
Submitted by Hamburg 2023

TAT-Ferritin

TAT-ferritin is a N-terminal fusion of the Trans Activator of Transcription (TAT) cell penetrating peptide (BBa_K1202006) to ferritin heavy chain (H. sapiens) (BBa_K4669000). Both parts are joined by a GS-linker (BBa_K4669003) to prevent potential steric hindrance of TAT by ferritin heavy chain. Under native conditions, ferritin heavy chain assembles into ferritin cages consisting of 24 subunits. The presence of TAT could thus confer cell penetrative properties to TAT-ferritin cages.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None