Part:BBa_K4633105

Bacillus subtilis L-arabinose induced killswitch

This composite construct features two distinct systems, the first is meant to allow for the expression of the araR protein, a repressor from Bacillus subtilis 168 that only allows for transcription in the presence of L-arabinose. The first system is composed of the native promoter – paraR (BBa_K4633004), followed by the native ribosomal binding site (RBS) (BBa_K4633005) and the protein araR (BBa_K4633003). Downstream this system is the T1 terminator (BBa_B0010) to ensure that the two systems won’t interfere with one another.

The second system is the inducible system, it is composed of paraE (BBa_K4633006), a promoter containing araR binding sites and thus can be inhibited by araR, its native RBS (BBa_K4633007) and lastly MazF (BBa_K302033), the chosen toxin, meant to induce bacterial demise.

Usage and Biology

This composite part is the complete killswitch system designed by the 2023 Technion team for our project.

To learn more about the killswitch we invite you to our wiki.

To learn more about the functionality of our basic parts we invite you to look at the parts pages here on the registry: paraE (BBa_K4633006), paraR (BBa_K4633004).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 186
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 213