Part:BBa_K4633005

RBS paraR - the RBS from the promoter for the araR gene from Bacillus subtilis 168

Usage and Biology

The RBS (Ribosome Binding Site) sequence provided here is designed to be used in conjunction with the promoter for the araR gene, a component of the L-arabinose operon in Bacillus subtilis. The araR gene encodes the repressor protein, and this RBS sequence is intended to facilitate the efficient translation of the araR mRNA into protein by enabling ribosome binding.

The first 5 bases of this RBS form the core recognition site for ribosome binding (GGAGG) [1]. Following these initial bases, there is a spacer sequence to ensure proper positioning of the ribosome and the start codon for translation initiation.

This specific RBS sequence has been tested and characterized in Bacillus subtilis 168, and its functionality has been assessed in the context of the araR promoter (BBa_K4633004).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization and Measurements

The functionality of this RBS sequence was evaluated by measuring its ability to facilitate efficient translation initiation in Bacillus subtilis 168. It was tested in combination with the araR promoter (BBa_K4633004) and mCherry (BBa_J06504) to ensure compatibility and effectiveness in driving protein expression.

References

• [1] Sá-Nogueira, I. & Mota, L. J. Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. J. Bacteriol. 179, 1598–1608 (1997).