Coding

Part:BBa_K4604013

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)


fluorescent protein mTurquoise

Fluorescent protein mTurquoise with an excitation maximum of 434 nm and emission maximum of 474 nm. Can be used together in a system with mVenus, another fluorescent protein, for FRET. This can be used to study protein-protein interactions. This BioBrick is mTurquoise with an N-terminal GS-linker to allow for fusion with another protein. In our case that was MazF, a toxin (BBa_K4604011). Also used and further characterized in BioBricks BBa_K4604017 and BBa_K4604018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None