Composite
Part:BBa_K4604017
Designed by: Hannah Swientek Group: iGEM23_Freiburg (2023-10-07)
piG_02a (leaky_tetR_riboK12_mazF_mTurq)
BioBrick piG_02a consits of the tet promoter/repressor, an AdoCbl riboswitch, mazF fused togther with mTurquoise and the rrnB terminator. The backbone we used in the experiments is pGGAselect. This version on the plasmid is leaky, a modified version of it is uploaded as BioBrick BBa_K4604018.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 727
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal AgeI site found at 1391 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2265
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Categories
Parameters
None |