Part:BBa_K4407015

Pthl-dps-aceE，Coexpression of dps and aceE with Pthl promoter

This part is responsible for co-expressing dps and aceE proteins under the control of a Pthl promoter and a terminator. It is composed of BBa_K3443002 (Pthl), BBa_K4407001 (dps), BBa_K4407002 (aceE), and BBa_K3585002 (terminator). dps protects the bacteria from oxidative damage. It protects DNA against oxidative damage by binding with Fe2+ to prevent it from reacting with H2O2 (Fenton’s reaction). aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment. In our project, we used this device to construct facultative anaerobic Clostridium tyrobutyricum (C. tyrobutyricum). This device combines the function of the two proteins, enhancing viability in aerobic environment by protecting DNA and substituting the main metabolic enzyme PFOR which is inactivated under aerobic condition with PDH in the presence of oxygen.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2349
  Illegal BglII site found at 2757
  Illegal BamHI site found at 1309
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3379
  Illegal AgeI site found at 2090
  
• 1000
  COMPATIBLE WITH RFC[1000]