Coding

Part:BBa_K4407001

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-Nanjing   (2022-09-30)


dps，DNA protection against oxidative damage

This part contains the coding region of the DNA-binding protein from starved cells (dps). dps gene is isolated from the genome of Deinococcus wulumuqiensis R12. dps protects the bacteria from oxidative damage. It can prevent the reaction of H2O2 with divalent iron ions (Fenton reaction) and the production of hydroxyl radicals, thus protecting DNA in the bacteria and increasing the cells’ tolerance in aerobic environments. In our project, we inserted dps gene into C. tyrobutyricum to make the bacteria facultative anaerobic.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None