Part:BBa_K4390109

Car9 tag expression (control)

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

Car9 is a short silica-binding tag to add to the N-terminal of a protein using JUMP assembly, including a short alanine-rich linker (AAAL). The tag facilitates immobilisation to silica surfaces with a dissociation constant (1 µM), enabling enzyme immobilisation or purification using silica-based spin columns. The advantage of using Car9 silica tag is its small size (1.87 kDa) would introduce smaller effect to the functional enzyme activity in theory (Coyle and Baneyx, 2014).

Reference

Coyle B, Baneyx F. A cleavable silica-binding affinity tag for rapid and inexpensive protein purification. Biotechnology and Bioengineering. 2014;111(10):2019-2026.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]