Part:BBa_K4390025

Si-tag :: Gly-Ser Linker

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a Level 0 part of type N-O part generating the following 4 base overhangs at upstream (AATG).

Usage and Biology

This is an NO part containing a silica-binding tag and a glycine-serine linker. The silica-binding tag allows for the expressed level 1 construct to bind to silica, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the silica tag interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to bind metallothioneins to silica beads. It does not have TTCG at the end to complete the sequence, as this is regenerated by the O part 3' fusion site in JUMP assembly. Add TTCG at the end to use as a normal part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 14
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]