Reporter
Part:BBa_K4244060
Designed by: Arthur Vancolen Group: iGEM22_Wageningen_UR (2022-10-09)
Constitutive expression of sfGFP with OmpA secretion tag
sfGFP BBa_K4244004 was fused to OmpA BBa_K208003 (fused at the N-terminus) to secrete sfGFP and use it as an extracellular reporter. It is under an constitutive medium strength promoter BBa_K4244057.
Secretion assay
Discussion
No secretion was observed in Escherichia coli Nissle 1917. This may be due to the too high expression level of sfGFP under BBa_K4244057. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |