Regulatory

Part:BBa_K4244057

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-09)


rbcL: Ribulose bisphosphate carboxylase large subunit

The sequences include the promoter and the 5-UTR. The sequences were amplified by PCR from the total DNA of the wild-type Phaeodactylum tricornutum CCAP 1055/1. Chloroplast promoters are eubacterial in origin and thus compatible with gene expression in bacteria [1,2].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Gatenby AA, Rothstein SJ, Bradley D. Using bacteria to analyze sequences involved in chloroplast gene expression. Photosynth Res. 1988;19(1–2):7–22. 2. Xie WH, Zhu CC, Zhang NS, Li DW, Yang WD, Liu JS, et al. Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum. Mar Biotechnol. 2014;16(5):538–46.

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