Coding

Part:BBa_K4244007

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)


GFP without BsaI

Silent mutations were performed to removed the BsaI site at position 664 (G to A), the NheI site at position 6 (T to A) and both AgeI sites at position 32 (C to G) and position 156 (C to G) present in BBa_E0040 to make it compatible with all assembly type.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None