Coding

Part:BBa_K4175012

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-09-25)


UAS-pSV40-CAR19-P2A-mCherry

This is a second-generation anti-CD19 chimeric antigen receptor (CAR) that comprises of a 4-1BB motif (BBa_K4175006) and a CD3ζ motif (BBa_K4175009). It is controlled by UAS-pSV40 promoter (BBa_K4040033), where UAS acts as a binding region for Gal4 while SV40 is a strong promoter. The anti-CD19 CAR is linked to the fluorescent protein, mCherry, by P2A self-cleavage peptide. This peptide will undergo ribosomal skipping upon translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1388
    Illegal NgoMIV site found at 1803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 949


[edit]
Categories
Parameters
biologyHuman