Regulatory
Part:BBa_K4040033
Designed by: Tong Yin Group: iGEM21_NMU_China (2021-09-29)
UAS-pSV40
This part (UAS-pSV40 ) is a mammalian synthetic promoter (SynPro) based on Sv40 promoter (BBa_K2446052) and 4*GAL4 binding sites from UAS-Gal4 Promoter MammoBlock(BBa_K511003 MIT2011) . We inset the 4*GAL4 binding sites before the Sv40 promoter. Sv40 promoter is a constitutive expression promoter from the simian vacuolating virus 40. The GAL4DBD can bind to the 4*GAL4 binding sites specifically and the KRAB domain can repress the expression of UAS SV40 promoter. The corresponding SynTF of UAS-pSV40 is ZF_GAl4_KRAB (BBa_K2446037).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |