Part:BBa_K4171015

PT7-tRNA(Sec)

Backgrounds

This is the DNA sequence of native tRNA^Sec (BBa_K4171008) of E.coli , which can bind with serine (Ser) by the help of enzyme SerRS (BBa_K4171001). With T7 promoter sequence (BBa_I719005) in the front, RNA polymerase is able to work properly on the template when doing in vitro RNA synthesis.

Fig. 1. In vitro Sec synthesis pathway

Usage

This sequence will be synthesized through in vitro RNA synthesis, which produces tRNA^Sec transcript, the substrate of SerRS for seryl-tRNA aminoacylation in tRNA selenocysteine (Sec) synthesis pathway.

Characterization

Fig. 2. Confirmation of P_T7 -tRNA^Sec (BBa_K4171015) constructed through PCR. M: Marker; Lane1: P_T7 -tRNA^Sec (118 bp)

Reference

[1]Fu X, Crnković A, Sevostyanova A, Söll D. Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s. FEBS Letters. 2018;592(22):3759-3768. doi:10.1002/1873-3468.13271

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]