Part:BBa_K4171014

PT7-tRNA(Ser)

Background

This is the DNA sequence of native tRNA^Ser (BBa_K4171007) of E.coli , which can bind with serine (Ser) by the help of enzyme SerRS (BBa_K4171001). With T7 promoter sequence (BBa_I719005) in the front, RNA polymerase is able to work properly on the template when doing in vitro RNA synthesis.

Fig. 1. In vitro Sec synthesis pathway

Usage

This sequence will be synthesized through in vitro RNA synthesis, which produces tRNA^Ser transcript, the substrate of SerRS for seryl-tRNA aminoacylation in tRNA selenocysteine (Sec) synthesis pathway.

Characterization

Fig. 2. Confirmation of P_T7 -tRNA^Ser (BBa_K4171014) constructed through PCR. M: Marker; Lane1: P_T7 -tRNA^Ser (111 bp)

Reference

[1]Asahara, H., Himeno, H., Tamura, K., Nameki, N., Hasegawa, T., & Shimizu, M. (1994). Escherichia coli SERYL-trna synthetase recognizes tRNASer by its characteristics tertiary structure. Journal of Molecular Biology, 236(3), 738–748. [2]https://parts.igem.org/Part:BBa_I719005

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 58
  
• 1000
  COMPATIBLE WITH RFC[1000]