Part:BBa_K3972006

5-UTR with g10-L RBS

To improve protein expression of any kind, a translation enhancing gene component can be added, additionally to the ribosome binding site. The addition of a 5’ untranslated region (5’-UTR) is already proven to increase expression of the consecutive gene, compared to the standard RBS BBa_B0034 [1]. The research paper of Volkenborn et. al confirms the importance of a 5’UTR sequence for translation initiation [2]. Part BBa_K3972006 is designed to optimally enhance protein expression in prokaryotes and it consists of translation enhancing DNA, a 10-A-spacer, a g10-L RBS, and an AT-rich region. This part is based on a different 5’UTR sequence (BBa_K1758100).

Usage and Biology

This part is used for the composite part (BBa_K3972005) and characterization results can be found on this page.

References

[1] Takahashi S, Furusawa H, Ueda T, Okahata Y. Translation enhancer improves the ribosome liberation from translation initiation. J Am Chem Soc. 2013;135(35):13096–106.

[2] Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A, Jaeger K-E. The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis. Microb Cell Fact. 2020;19(1):154.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]