Composite

Part:BBa_K3933019

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-21)


Standardized version of our sensor to be adapted for riboswitches inducing the translation

This part was developed for our implemented Human Practice, so that other teams could be able to adapt our system to the molecule they want to detect. This construction contains a Riboswitch Insertion Zone (RIZ), which contains restriction enzymes sites. Thanks to these sites, this construction can be digested and used for Golden Gate Assembly or Restriction Enzyme assembly, to add a riboswitch in lieu of the RIZ. This construction is to be used when the activation of the riboswitch - that is to say when it binds the molecule to detect - triggers the translation of the coding sequence.

BBa K3933019 schema.png

This part is composed of a constitutive promoter (BBa_J23106), followed by the RIZ (BBa_K3933010). After is the signalling unit, composed of a RBS (BBa_B0032), a Lactate dehydrogenase (BBa_K3102006) and a double terminator (BBa_B0015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 54
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 393


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