Reporter

Part:BBa_K3889002

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-02)


modified_sfGFP

sfGFP is a variant of Green Fluorescence Protein from Aequorea victoria and has the following parameters [1]:

Ex : Peak excitation wavelength (in nm) 485
Em: Peak emission wavelength (in nm) 510
EC: Extinction Coefficient (M-1 cm-1) 83,300
QY: Quantum Yield 0.65
Brightness 54.15
Maturation (min) 13.6


Usage and Biology

sfGFP can be used to report protein production or in systems where fluorescence is required like FRET. In BBa_K3889102, BBa_K3889110 and BBa_K3889122 we planned to use sfGFP as a reporter for ovastacin, xylR and mf-Lon production respectively in Bacillus subtilis 168. In BBa_K3889130, sfGFP along with BBa_J06504 for checking terminator efficiency in Bacillus subtilis 168.

This is a modified version of BBa_I746916 specific to our project's assembly. Mutated Kpn1 site and changed stop codon to make assembly compatible part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13



References

1. https://www.fpbase.org/protein/superfolder-gfp/

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Categories
Parameters
None