Composite

Part:BBa_K3889102

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-08)


Ovastacin expression cassette Bacillus subtilis

P43 (BBa_K143013) fused with P22 binding site L (BBa_K3889091) and RBS (BBa_K1351030) in order to create P22 c2 repressed Ovastacin expression. Also in order to secrete Ovastacin PhoD signal peptide (BBa_K3889050) was also fused.

Usage and Biology

This part along with BBa_K3889100 and BBa_K3889101 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None