Regulatory

Part:BBa_K3848002

Designed by: Shakti Virendra dahe   Group: iGEM21_IISER_Bhopal   (2021-09-23)


AND gate - Hypoxia promoter and Lactate operator

This part consists of a hypoxia-inducible promoter (BBa_K387003) intercalated between lactate operators (BBa_K1847008). The hypoxia promoter works under the hypoxic environment, and it has a much higher efficiency of starting transcription than under normal 20% oxygen pressure. The operator regulates the expression of llPRD operon and is involved in L-lactate metabolism. The promoter is repressed by a dimer of LldR, possibly by forming a DNA loop that does not allow the RNA polymerase to bind to the promoter. LldR can also have a function as an activator [1]. The repression of the promoter is removed by lactate, as it binds to LldR and prevents the looping of DNA.


References

Aguilera L, Campos E, Giménez R, Badía J, Aguilar J, Baldoma L. Dual role of LldR in regulation of the lldPRD operon, involved in L-lactate metabolism in Escherichia coli. J Bacteriol. 2008 Apr;190(8):2997-3005. doi: 10.1128/JB.02013-07. Epub 2008 Feb 8. PMID: 18263722; PMCID: PMC2293229.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None