Coding

Part:BBa_K3793005

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


CDS - Human AhR Nuclear Translocator (Truncated)

This sequence encodes for the truncated version of the human Aryl hydrocarbon receptor Nuclear Translocator(ARNT). ARNT is the dimerizing partner of Aryl hydrocarbon receptor (AhR). The heterodimer of AhR-ARNT function as a transcription factor in a ligand-activated fashion. Upon ligand binding to AhR, it gets translocated to the nucleus where it dimerizes with ARNT. The AhR-ARNT heterodimer binds to the binds to the DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription.

The part contains the coding sequence for a truncated version of ARNT containing the the basic helix-loop-helix, PAS-A and PAS-B domains. The CDS has been codon optimized to remove any type IIS restriction enzyme sites. An N-Terminal 6x HIS tag is added to the CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 104
    Illegal EcoRI site found at 1210
    Illegal SpeI site found at 1054
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 104
    Illegal EcoRI site found at 1210
    Illegal SpeI site found at 1054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 104
    Illegal EcoRI site found at 1210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 104
    Illegal EcoRI site found at 1210
    Illegal SpeI site found at 1054
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 104
    Illegal EcoRI site found at 1210
    Illegal SpeI site found at 1054
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None