Composite

Part:BBa_K3746008

Designed by: Cheung Chi Ming   Group: iGEM21_Hong_Kong_JSS   (2021-10-18)


T7-tvLac-6His

Description

This is a part design for the Phase I project (2021 iGEM) of Team Hong Kong JSS.

The constitutively expression construct of laccase from Trametes versicolor (tvLac) (BBa_K3746002).

The coding sequence of tvLac is driven by T7 promoter (BBa_I719005), a strong RBS (BBa_K3076801), and a T7 terminator (BBa_K395601).

The laccase protein is tagged with a 6-His Tag (CATCATCATCATCATCAT) for protein purification.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 53
    Illegal PstI site found at 1163
    Illegal PstI site found at 1206
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 53
    Illegal PstI site found at 1163
    Illegal PstI site found at 1206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1561
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 53
    Illegal PstI site found at 1163
    Illegal PstI site found at 1206
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 53
    Illegal PstI site found at 1163
    Illegal PstI site found at 1206
    Illegal AgeI site found at 145
    Illegal AgeI site found at 958
    Illegal AgeI site found at 1388
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None