Composite

Part:BBa_K3657032

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


MS2 Coat Protein coupled with GFP

This is a fusion protein containing MS2 Coat Protein (BBa_K3218006) and GFP (BBa_E0040).


Usage and Biology

MS2 Coat Protein binds specifically the MS2 RNA hairpin (BBa_K3113006).

GFP is a green fluorescent protein


This construct was planned to be used as the positive controle in an experiment aiming to explore the ability to use RNA as a linker between proteins. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1049


[edit]
Categories
Parameters
None