Project

Part:BBa_K329068

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-25)


Population counter Tetracycline resistant - PsS-WTet-LuxI

This part is one of our population counters. When IntI1 (BBa_K329014) is expressed, it excises the double terminator flanked by attC sites (BBa_J99001) and triggers the expression of TetA(C) (with a weak RBS, BBa_K329004) and LuxI (BBa_K081008) because of the constitutive promoter upstream.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1295
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3006
    Illegal BamHI site found at 1441
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1467
    Illegal NgoMIV site found at 1835
    Illegal NgoMIV site found at 1995
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None