Translational_Unit

Part:BBa_K329014

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-24)

RBS - IntI1

IntI1 is an integron integrase of the class 1 multiresistant integron (MRI). The integron platform consists of an integrase gene (intI) belonging to the tyrosine recombinase family and a primary recombination site, attI. The integrase mediates recombination between the attI site and a target recombination sequence called an attC site (or 59-base element). The attC site is usually found associated with a single open reading frame in a circularized structure termed a gene cassette. Insertion of the gene cassette at the attI site, which is located downstream of a resident promoter, Pc, internal to the intI gene, drives expression of the encoded proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 245


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Categories
Parameters
None