Part:BBa_K3237019
His-mRFP1-SCI57 with furin cut sites codon optimized for C. reinhardtii
The SCI57 insulin protein is a single chain insulin variant that has been found to be ultra stable [1]. being that it is very stable, we have chosen it as a candidate for studies in expressing and deploying insulin in microalgae. Along with the coding sequence for this single chain insulin, we have also fused it to mRFP1 also known as BBa_E1010 for expression standardization purposes. In between this fusion is a furin cut site [2] and a linker in order to remove the RFP once in contact with the human protease. The other component added is a hexahistidine tag added to the N-terminus of the construct for purification purposes. This part is codon optimized for the microalgae species Chlamydomonas reinhardtii . For our project we had made a counterpart construct optimized for E.coli BBa_K3237005.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1700
Illegal XbaI site found at 1688
Illegal XbaI site found at 1730
Illegal SpeI site found at 1724
Illegal PstI site found at 1238
Illegal PstI site found at 1706 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1700
Illegal NheI site found at 1252
Illegal SpeI site found at 1724
Illegal PstI site found at 1238
Illegal PstI site found at 1706
Illegal NotI site found at 1736 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1700
Illegal BamHI site found at 1718
Illegal XhoI site found at 656 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1700
Illegal XbaI site found at 1688
Illegal XbaI site found at 1730
Illegal SpeI site found at 1724
Illegal PstI site found at 1238
Illegal PstI site found at 1706 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1700
Illegal XbaI site found at 1688
Illegal XbaI site found at 1730
Illegal SpeI site found at 1724
Illegal PstI site found at 1238
Illegal PstI site found at 1706 - 1000COMPATIBLE WITH RFC[1000]
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