Part:BBa_K3237007
His-mRFP1-SCI57-CPP with furin cut sites codon optimized for E.coli
This part is an expression construct for SCI57, a super-stable single chain insulin variant [1]. This part is fused to a hexahistidine tagged mRFP1 on the N-terminus and a protein transduction domain (that acts as a cell penetrating peptide) on the C-terminus. The mRPF1 is based on the registry part BBa_E1010. The protein transduction domain was added as it has been shown to improve insulin uptake into the intestinal wall, allowing for increased introduction into the bloodstream from the GI tract [2]. Furin cut sites have been added between the mRFP1-SCI57 fusion and the SCI57-Protein transduction domain fusion to allow for removal as the protein enters the blood stream as furin is a human specific protein [3] or during in vitro studies. This part was used for comparison purposes as our main project was to express the insulin in microalgae.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 588
Illegal AgeI site found at 700 - 1000COMPATIBLE WITH RFC[1000]
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