Composite

Part:BBa_K316025

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-27)

Dif-PmeI site with 3amyE


This is the 3' part of B. subtilis genome integration vectors BBa_K316022 and BBa_K316027. The 3' amyE integration sequence BBa_K143002 for integration into B.subtilis genome was combined with a dif and PmeI recognition site BBa_K316014.


Vectordif3.PNG


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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