Intermediate

Part:BBa_K316014

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)

Dif sequence followed by PmeI recognition site


This composite part of BBa_K143000 and BBa_K143013. The dif site can be used in conjunction with another dif site in another part of the vector to remove a sequence between the two dif sites. PmeI site can be used for blunt end cloning of a DNA sequence behind the dif site.


Please see ‘Part Design’ section for design considerations and parts used.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None