Part:BBa_K316019

Reverse strand coding ComE responsive promoter

This part is the 3' coding version ofthe BioBrick BBa_K316018. ComE BBa_K316016 binds to a specific sequence in a promoter leading to activation of transcription downstream of the binding site. This sequence regulates the ComE responsive promoter in the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within S. pneumoniae[http://www.ncbi.nlm.nih.gov/pubmed/17328748].

Figure I. ComE binding sites in S. pneumonia genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]

For more information about ComCDE system, please check our wiki [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

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1. 1 A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)[http://www.ncbi.nlm.nih.gov/pubmed/17328748]

</biblio>