Generator
ComE

Part:BBa_K316016

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-20)

ComE transcription factor phosphorylation-activated by ComD


ComE is the response regulator of a two-component signal transduction signaling pathway in a Streptococcus pneumoniae quorum sensing system. It is activated when a linear peptide ligand binds to ComD BBa_K316015 . On binding, ComD autophosphorylates and transfers the phosphate to ComE. Phosphorylated ComE can bind to DNA and dimerise, thus activating transcription at specific binding regions within promoters. Biobricks BBa_K316018 and BBa_K316018 contain 5' and 3' coding promoter with a ComE binding region respectively.


ComEbs.PNG

Figure I. ComE binding sites in S. pneumonia genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]


For more information on ComCDE system, please check our [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling wiki]


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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