Composite

Part:BBa_K2983005

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-11)


Ura3 auxotrophic selection marker

This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.

Usage and Biology

The URA3 auxotrophic selection marker (BBa_K2983005) is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004).

The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.

It is a wildly used auxotrophic selection marker in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 195
    Illegal XhoI site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 486
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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