This part is the Ura3 gene of Yarrowia lipolytica.
Usage and Biology
The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 126.96.36.199) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.
It is a wildly used auxotrophic selection marker in yeast.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal XhoI site found at 17
Illegal XhoI site found at 50
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 205
Illegal AgeI site found at 308
- 1000COMPATIBLE WITH RFC