RNA

Part:BBa_K2810003

Designed by: Geraint Parry   Group: iGEM18_Cardiff_Wales   (2018-08-24)

Full sequence for Myzus persisae BCR3 secreted protein

BCR3 is a predicted secreted protein that localizes to aphid bacteriocyte cells.

This is the full coding sequence from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi.

BCR3_full is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the Phytobrick standard.

It is flanked by BsaI sites together 5' TACT and 3' CCAT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K2810004.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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