Reporter

Part:BBa_K2810001

Designed by: Geraint Parry   Group: iGEM18_Cardiff_Wales   (2018-08-24)


GUS reporter for plant expression

The GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.

The GUS gene is cloned into the golden gate version of pSB1C3 (BBa_P10500) using BsmBI/Esp3I and conforms to the Phytobrick standard. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Composite Parts page.

Using this part, the Cardiff iGEM team of 2018 characterised 35S CaMV promoter, the RTBV promoter, Nopaline synthase terminator, 35S CaMV terminator, and G7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None