Coding

Part:BBa_K2719010

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)


Leptin coding device with blue chromoprotein

This part facilitates the selection of transformed colonies for the production of leptin (BBa_K2719009) by the incorporation of a reporter cassette (BBa_K592015), which constitutively expresses blue chrome protein. This reporter simplifies the selection of colonies by the appearance of blue colored colonies, serving as transformation and ligation control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None