Device

Part:BBa_K2719009

Designed by: Andrea Pamela Jimenez Tapia & Rodrigo Valencia Ocampo   Group: iGEM18_TecCEM   (2018-08-08)


Leptin Coding Device

This part is designed for the expression of leptin in E.coli BL21 (DE3), for that function it consists on a T7-lac promoter (BBa_R0184), RBS (BBa_J611000), the leptin coding site (BBa_K2719008) and a double terminator (BBa_B0015). This part was synthetized and the sequence was designed using the standard BioBrick assembly, therefore it has the BioBrick suffix and prefix. This part allows for an inducible expression of recombinant leptin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None