Composite

Part:BBa_K2680258

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G LacI-ssrA

BBa_K2680538 flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

BBa_K2680538 is LacI preceded by a two-basepair scar and followed by an ssra degradation tag.

"LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds to LacI and inhibits its operation, therefore promoting transcription."1

References

1Kenney, G., Shen, D., Varshney, N., & Sutton, S. (2003, January 31). Part:BBa_C0012. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_C0012


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 1190


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