Coding

Part:BBa_K2680538

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-15)


LacI-ssra

LacI preceded by a two-basepair scar and followed by an ssra degradation tag.

"LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds to LacI and inhibits its operation, therefore promoting transcription."1

References

1Kenney, G., Shen, D., Varshney, N., & Sutton, S. (2003, January 31). Part:BBa_C0012. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_C0012

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None