Regulatory

Part:BBa_K2448014

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)


pPsiA from Sinorhizobium meliloti

This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium meliloti (SMb20484)

pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiA promoter together with the Sinorhizobium meliloti PsiR transcription factor (BBa_K2448008) to build a Psicose biosensor (BBa_K2448030). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction. 

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    229 LDF-  0.81
-10 box at pos.    214 ctgaatgat Score    31
-35 box at pos.    197 ttgacg    Score    60
Oligonucleotides from known TF binding sites:
No such sites for promoter at    229

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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