pPsiA from Sinorhizobium meliloti
This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium meliloti (SMb20484)
pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose .
Usage and Biology
Our team used this pPsiA promoter together with the Sinorhizobium meliloti PsiR transcription factor (BBa_K2448008) to build a Psicose biosensor (BBa_K2448030). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction.
Using the BPROM webserver , the predicted features of this promoter are: Threshold for promoters - 0.20 Number of predicted promoters - 1 Promoter Pos: 229 LDF- 0.81 -10 box at pos. 214 ctgaatgat Score 31 -35 box at pos. 197 ttgacg Score 60 Oligonucleotides from known TF binding sites: No such sites for promoter at 229
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 344
- 1000COMPATIBLE WITH RFC