PsiR from Sinorhizobium fredii
This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).
Usage and Biology
PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose . This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).
Our team used this transcription factor together with the Sinorhizobium fredii pPsiA or pPsiR promoters (BBa_K2448012 and BBa_K2448013) to build two Psicose biosensors (BBa_K2448028 and BBa_K2448029). The results show that PsiR behaved as predicted under and without induction.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 691
Illegal AgeI site found at 1024
- 1000COMPATIBLE WITH RFC