Regulatory
WRKY30

Part:BBa_K2404005

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)


WRKY30 - a promoter induced by the presence of cellulose-derived oligomers

The WRKY30 promotor is a regulatory sequence from Arabidopsis thaliana that has been characterised as a [http://www.plantphysiol.org/content/early/2017/02/27/pp.16.01680 ‘Damage Associated Molecular Patterns’ (DAMP)].

This promotor is situated -653 > -168 upstream of the WRKY30 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 122
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 477
    Illegal BsaI.rc site found at 502


[edit]
Categories
Parameters
None